ALLEVIATION OF SALINITY STRESS IN GROUNDNUT BY APPLICATION OF PGPR

Abstract

Two high salt tolerant Plant Growth Promoting bacteria(BM6, AMAAS57) were isolated from Gujarat kuttch region. Their PGP characters were characterized at lab by different biochemical test. To study their ability to promote growth of plant under salinity, pot trial was conducted using groundnut. Different physiological parameters (carbohydrate, phenol and free amino acid, SLA, RWC) were studied. Two pseudomonas culture having plant growth promoting tratits like production of IAA, HCN, ammonia, phosphate solubilisation, antifungal activity and tolerant to salinity (10% NaCl). These cultures were identified by 16s rRNA sequencing viz. Pseudomonas aeruginosa AMAAS57 and Pseudomonas aeruginosa BM6. Application of Pseudomonas aeruginosa AMAAS57 increased the production of phenol and free amino acids with soil salinity of 2 ds/m but thereafter decreased gradually with increasing salinity the decrease as compared to control. application of Pseudomonas aeruginosa AMAAS57 lowered the level of RWC% with increase in salinity. Application of Pseudomonas aeruginosa AMAAS57 and Pseudomonas fluorescens BM6 reduced the electrolyte leakage.