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Title: | Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India. |
Other Titles: | Not Available |
Authors: | Kumar R. Kushwaha B. Nagpure N.S. Behera B.K. Lakra W.S. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources ICAR::Central Inland Fisheries Research Institute ICAR::Central Institute of Fisheries Education |
Published/ Complete Date: | 2013-08 |
Project Code: | Not Available |
Keywords: | Channa spp cytogenetics Manipur nucleolar organizer region rDNA |
Publisher: | Taylor & Francis |
Citation: | Kumar R., Kushwaha B., Nagpure N.S., Behera B.K., and Lakra W.S. 2013. Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India. Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics, 66 (2):109-119.DOI: 10.1080/00087114.2013.821829 |
Series/Report no.: | Not Available; |
Abstract/Description: | Genetic variability was assessed among three species of genus Channa, namely C. gachua, C. punctata and C. striata,using karyological tools including karyomorphology, silver nitrate and chromomycin A3 staining of nucleolar organizerregions (NORs) and C-banding. Additionally, sequence variation in partial 18S and divergent domain 1 of 28S ribosomal DNA was utilized for inter-species diversity assessment. Diploid chromosome numbers of 32, 40 and 52 in the three species were observed and the karyotype formula was derived as 18m + 12sm + 2st (fundamental arm number [FN] = 62); 6m + 2sm + 10st + 22t (FN = 48) and 12m + 10sm + 14st + 16t (FN = 86) in C. punctata, C. striata and C. gachua, respectively. Similarly, variations were also observed in number and location of silver and chromomycin A3 stained NORs and C-banding patterns. DNA sequencing generated sizes for 18S of 243, 248, 245 bp and for 28S of 401, 392 and 393 bp in C. gachua, C. punctata and C. striata, respectively. The results indicated that the cytogenetic and molecular markers are useful for the assessment of genetic diversity among the fish species and could provide valuable information for germplasm evaluation and ex situ conservation. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Caryologia |
NAAS Rating: | 6.62 |
Volume No.: | 66 (2) |
Page Number: | 109-119 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI: 10.1080/00087114.2013.821829 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/16060 |
Appears in Collections: | FS-CIFRI-Publication |
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