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http://krishi.icar.gov.in/jspui/handle/123456789/16337| Title: | Multi track staining apparatus with high resolution and time-lapsed acquisition for polyacrylamide gels. |
| Other Titles: | Not Available |
| Authors: | Maurye P. Basu A. Biswas J.K. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Inland Fisheries Research Institute |
| Published/ Complete Date: | 2015-03-18 |
| Project Code: | Not Available |
| Keywords: | polyacrylamide gel proteins nucleic acids silver staining |
| Publisher: | Taylor & Francis |
| Citation: | Maurye P., Basu A. and Biswas J.K. 2015. Multi track staining apparatus with high resolution and time-lapsed acquisition for polyacrylamide gels. Instrumentation Science & Technology. DOI: 10.1080/10739149.2015.1027919. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Polyacrylamide gel electrophoresis (PAGE) is commonly used for the separation, identification, and purification of mixtures of proteins and nucleic acids. A suitable staining method for the polyacrylamide gels is imperative to determine low concentrations of resolved macromolecules. Several staining methods have been adopted; ethidium bromide, silver, and coomassie staining are the most frequently employed. Silver staining determines macromolecules at nanomolar concentrations but involves many steps and thus is tedious for multiple gels in parallel. In this article, a simple multitrack laboratory-constructed silver staining apparatus is characterized that may enhance the sensitivity of the electrophoresis stains and the homogeneity of detection. In addition, the low cost and simplicity of this system provide wide applicability for gel electrophoresis. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal Instrumentation Science & Technology |
| Volume No.: | 43(5) |
| Page Number: | 588-600 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1080/10739149.2015.1027919 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/16337 |
| Appears in Collections: | FS-CIFRI-Publication |
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