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http://krishi.icar.gov.in/jspui/handle/123456789/16464
Title: | RNA-seq of Rice Yellow stem borer Scirpophaga incertulas reveals molecular insights during four larval developmental stages. 2017. |
Other Titles: | Not Available |
Authors: | P. Renuka, Maganti S. Madhav, A. P. Padmakumari, Kalyani M. Barbadikar, Satendra K. Mangrauthia, K. Vijaya Sudhakara Rao, Soma S. Marla, and V. Ravindra Babu |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR- Indian Iinstitute of Rice research |
Published/ Complete Date: | 2017-07-17 |
Project Code: | Department of Biotechnology, Government of India under grant BT/PR2468/AGR/36/701/2011 dt 23.05.2012. |
Keywords: | insec,t Scirpophaga incertulas, de novo transcriptome, RNAi growth and development, detoxification mechanism |
Publisher: | Genetics Society of America |
Citation: | Pichili Renuka,* Maganti S. Madhav,* Ayyagari Phani Padmakumari, Kalyani M. Barbadikar,* Satendra K. Mangrauthia,* Kola Vijaya Sudhakara Rao,* Soma S. Marla, ‡ and Vemuri Ravindra Babu.2017.RNA-seq of Rice Yellow Stem Borer Scirpophaga incertulas Reveals Molecular Insights During Four Larval Developmental Stages. genes, genomes and genetics .https://doi.org/10.1534/g3.117.043737 |
Series/Report no.: | Not Available; |
Abstract/Description: | ABSTRACT The yellow stem borer (YSB), Scirpophaga incertulas, is a prominent pest in rice cultivation causing serious yield losses. The larval stage is an important stage in YSB, responsible for maximum infestation. However, limited knowledge exists on the biology and mechanisms underlying the growth and differentiation of YSB. To understand and identify the genes involved in YSB development and infestation, so as to design pest control strategies, we performed de novo transcriptome analysis at the first, third, fifth, and seventh larval developmental stages employing Illumina Hi-seq. High-quality reads (HQR) of 229 Mb were assembled into 24,775 transcripts with an average size of 1485 bp. Genes associated with various metabolic processes, i.e., detoxification mechanism [CYP450, GSTs, and carboxylesterases (CarEs)], RNA interference (RNAi) machinery (Dcr-1, Dcr-2, Ago-1, Ago-2, Sid-1, Sid-2, Sid-3, and Sid-1-related gene), chemoreception (CSPs, GRs, OBPs, and ORs), and regulators [transcription factors (TFs) and hormones] were differentially regulated during the developmental stages. Identification of stage-specific transcripts made it possible to determine the essential processes of larval development. Comparative transcriptome analysis revealed that YSB has not evolved much with respect to the detoxification mechanism, but showed the presence of distinct RNAi machinery. The presence of strong specific visual recognition coupled with chemosensory mechanisms supports the monophagous nature of YSB. Designed expressed sequenced tags-simple-sequence repeats (EST-SSRs) will facilitate accurate estimation of the genetic diversity of YSB. This is the first report on characterization of the YSB transcriptome and the identification of genes involved in key processes, which will help researchers and industry to devise novel pest control strategies. This study also opens up a new avenue to develop next-generation resistant rice using RNAi or genome editing approaches. |
Description: | larval transcriptome of yellow stem borer was studied |
ISSN: | Online ISSN: 2160-1836 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | G3 - Genes Genomes Genetics |
NAAS Rating: | 8.78 |
Volume No.: | 7 |
Page Number: | 3031-3045 |
Name of the Division/Regional Station: | entomology, Biotechnology |
Source, DOI or any other URL: | https://doi.org/10.1534/g3.117.043737 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/16464 |
Appears in Collections: | CS-IIRR-Publication |
Files in This Item:
File | Description | Size | Format | |
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3031.full G 3published .pdf | 2.35 MB | Adobe PDF | View/Open |
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