Genotypic comparison for androgenic callogenesis and organogenesis among cultivated, wild and interspecific hybrid of groundnut

Abstract

Anther or microspore culture is one of the efficient techniques for invitro plant regeneration and has been exploited in haploid breeding. Six groundnut cultivars, five wild Arachis species and four synthetic interspecific hybrids were studied for invitro androgenic callogenesis and regeneration. Androgenic callogenesis in roundnut starts four to five days after culturing in MS salts with vitamins of B5+12.362μM NAA+2.22μM BAP+87.64μM sucrose (m/v) and 0.6% agar (m/v) irrespective of ecotype. Callogenesis ranged from 21% to 72% among the genotypes studied. Cultivated genotypes were more responsive to callogenosis than wild species and interspeicific hybrids. Organogenesis induced in half the strength MS salts with vitamins of B5+6.66mM BAP+2.68μM NAA+2.89μM GA3 +87.64μM sucrose (m/v) and 0.6% agar (m/v). Four cultivated genotypes viz., GG 2, J11, JL 24 and TMV 2 induced shoot development and plants were regenerated from cultivars GG 2 and J 11. Regenerated plants were confirmed as 2n = 4x = 40 by root tip analysis indicating that regenerated plants were regenerated either from sporophytic tissue of microspore or spontaneous doubled haploid.