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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/18053
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Not Available | en_US |
dc.date.accessioned | 2019-04-06T11:35:35Z | - |
dc.date.available | 2019-04-06T11:35:35Z | - |
dc.date.issued | 2015-09-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/18053 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The objective of this study was to identify and characterize sequences from watermelon related to drought tolerant genes. In order to achieve this, publicly available Cucumis melo REB gene (Cme-DREB1) sequence was retrieved from NCBI database and gene-specific primers were synthesized. A fragment of 274 bp corresponding to DREB-like gene was amplified from genomic DNA of watermelon. PCR product was cloned in E. coli vector (pDrive) and validated through sequencing. The BLAST search showed 99 and 97% homology to CmeDREB1 gene and C. sativus DREB3-like (CsDREB3-like) gene, respectively. Furthermore, it showed 87 to 93% homology with rest of DREB genes available in the public database. Full length watermelon DREB-like gene (C. lanatus DREB-like, ClaDREB-like) was identified from cucurbit genomic database and investigated through in silico analysis. The intronless structural organization of ClaDREB-like gene was found with a conserved AP2/ERF domain. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Indian Society of Horticulture New Delhi | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Drought tolerance | en_US |
dc.subject | DREB | en_US |
dc.subject | in silico analysis | en_US |
dc.subject | watermelon | en_US |
dc.title | Molecular cloning and in silico analysis of DREB-like gene in watermelon | en_US |
dc.title.alternative | Chet Ram , Manjusha Verma, Bhupendra Singh Panwar and Lalit Arya | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Horticulture | en_US |
dc.publication.volumeno | 72(3) | en_US |
dc.publication.pagenumber | 2015: 338-342 | en_US |
dc.publication.divisionUnit | Division of Crop Improvement | en_US |
dc.publication.sourceUrl | DOI : 10.5958/0974-0112.2015.00066.3 | en_US |
dc.publication.authorAffiliation | ICAR:Central Institute for Arid Horticulture | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.16 | en_US |
Appears in Collections: | HS-CIAH-Publication |
Files in This Item:
File | Description | Size | Format | |
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Molecular cloning and in silico analysis of DREB-like gene in watermelon.pdf | 413.83 kB | Adobe PDF | View/Open |
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