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http://krishi.icar.gov.in/jspui/handle/123456789/1894
Title: | A simple model for selection and rapid advancement of transgenic progeny in sorghum |
Other Titles: | Not Available |
Authors: | K. B. R. S. Visarada E.N. Saikishore S. V. Kuriakose V. Shobha Rani M. Royer S. V. Rao N. Seetharama |
Published/ Complete Date: | 2008-07-25 |
Project Code: | Not Available |
Keywords: | Bar Correlation Leaf paint Transgenic breeding |
Publisher: | Springer |
Citation: | K. B. R. S. Visarada, N. Saikishore, S. V. Kuriakose, V. Shobha Rani, M. Royer, S. V. Rao, N. Seetharama (2008) A simple model for selection and rapid advancement of transgenic progeny in sorghum. Plant Biotechnology Reports 2 (1):47-58 |
Series/Report no.: | Not Available; |
Abstract/Description: | To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct in planta assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of bar gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for cry1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, twostep scheme for effective evaluation of transgenics and their progeny containing bar gene as selection marker using BASTA swab tests is described. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Plant Biotechnology Reports |
NAAS Rating: | 7.46 |
Volume No.: | 2 |
Page Number: | 47-58 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | http://link.springer.com/article/10.1007/s11816-008-0048-5 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/1894 |
Appears in Collections: | CS-IIMilletsR-Publication |
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