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http://krishi.icar.gov.in/jspui/handle/123456789/21890| Title: | In vitro Callusing and Regeneration in Trifolium resupinatum—A Fodder Legume |
| Other Titles: | Not Available |
| Authors: | D. R. Malaviya A. K. Roy Aparna Tiwari P. Kaushal Bijendra Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Indian Grassland and Fodder Research Institute, Jhansi-284003 (India) |
| Published/ Complete Date: | 2006-09-01 |
| Project Code: | Not Available |
| Keywords: | Trifolium resupinatum Fodder Regeneration Tissue culture |
| Publisher: | The Japan Mendel Society |
| Citation: | Devendra R. Malaviya, Ajoy K. Roy, Aparna Tiwari, Pankaj Kaushal and Bijendra Kumar.2006. In vitro Callusing and Regeneration in Trifolium resupinatum—A Fodder Legume. Cytologia 71(3): 229–235. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | In vitro regeneration protocol was developed in Trifolium resupinatum, an important fodder legume in Northwestern India. Out of 6 explants (leaf, petiole, hypocotyl, cotyledon, collar and root) tried for callus induction in 2 different media, good response was observed from hypocotyl and root explants in ‘A’ medium. Successful regeneration was obtained from callus induced from hypocotyl, cotyledon and root in ‘A’ followed with subculture in ‘E’ medium. One of the regenerated plantlet was characterized and compared with mother plant for isozyme-banding pattern. Wide variation for isozyme banding pattern for SOD, Peroxidase and GOT enzyme indicates the regenerant to be a somaclonal variant. Thus, in vitro regeneration will help in widening the genetic base of the species. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Cytologia |
| NAAS Rating: | 6.8 |
| Volume No.: | 71(3) |
| Page Number: | 229 - 235 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://square.umin.ac.jp/mendel/JournalENGLISH/2006September1.html |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/21890 |
| Appears in Collections: | CS-IGFRI-Publication |
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