KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/22947
Title: | GC-MS Fingerprinting of Fatty Acids of Freshwater Mollusc Lamellidens Marginalis using Different Columns, TR-WAX and TR-FAME. |
Other Titles: | Not Available |
Authors: | Mahanty A, Maji SR, Ganguly S, Mohanty BP |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Inland Fisheries Research Institute Bose Institute |
Published/ Complete Date: | 2015-02-10 |
Project Code: | Not Available |
Keywords: | Lipid biomarkers; Fatty acid; GC-MS fingerprinting; Lamellidens marginalis; Pollution monitoring |
Publisher: | Not Available |
Citation: | Mahanty A, Maji SR, Ganguly S, Mohanty BP (2015). GC-MS Fingerprinting of Fatty Acids of Freshwater Mollusc Lamellidens Marginalis using Different Columns, TR-WAX and TR-FAME. Analytical and bioanalytical Techniques 6:2. |
Series/Report no.: | Not Available; |
Abstract/Description: | GC-MS is an important instrument in lipid profiling and lipid biomarker identification. The choice of columns and detectors is important in analysis and identification of the biomolecules. In the present study, GC-MS fingerprinting of fatty acids (FAs) in different tissues of freshwater mollusc Lamellidens marginalis was carried out in GC-MS with two different columns, TR-FAME and TR-WaxMS, to examine if choice of columns and detectors influence the identifications of the lipids. These sentinel organisms are used in aquatic pollution monitoring studies as they accumulate toxic environmental contaminants to levels well above those present in the surrounding environment thus providing information on the spatio-temporal pollution trends. FAs identified in different tissues include the saturated FAs palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0), monounsaturated FAs (MUFAs) oleic acid (C18:1), eicosenoic acid (C20:1) and polyunsaturated fatty acid (PUFAs) linoleic acid (C18:2). More numbers of FAs were detected in TR-FAME column than TR-WaxMS column. Several non-fatty acid compounds like butylated hydroxyl toluene (BHT) and dibutyl phthalate were also identified in the FAME (fatty acid methyl ester) preparation using NIST (MS) library. It was interesting to note that the FA peaks (6/14) were outnumbered by the non-fatty acid peaks (8/14). Such compounds could be taken as unidentified FAs unless powerful detectors like MS are available and can lead to erroneous inferences. The study showed that the choice of columns and detectors influenced the identification of the compounds. The information generated on fatty acid profiles of this bivalve could serve as baseline data for this species in searching for lipid biomarkers for aquatic pollution monitoring studies. |
Description: | Not Available |
ISSN: | ISSN: 2155-9872 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Analytical and bioanalytical Techniques |
Volume No.: | 6 |
Page Number: | 238 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI: 10.4172/2155-9872.1000238 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/22947 |
Appears in Collections: | FS-CIFRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.