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http://krishi.icar.gov.in/jspui/handle/123456789/22948| Title: | DNA barcoding Indian freshwater fishes. |
| Other Titles: | Not Available |
| Authors: | Lakra W.S, Singh M., Goswami M., Gopalakrishnan A., Lal K.K. Mohindra V., Sarkar U. K. Punia P. Singh, K.V. Bhat J.P. Ayyappan S. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Inland Fisheries Research Institute ICAR::Central Institute of Fisheries Education ICAR::National Bureau of Fish Genetic Resources HNB Gharwal University Indian Council of Agricultural Research |
| Published/ Complete Date: | 2015-12-01 |
| Project Code: | Not Available |
| Keywords: | Barcoding, COI, Indian freshwater fishes, mtDNA |
| Publisher: | Not Available |
| Citation: | Lakra W.S, Singh M., Goswami M., Gopalakrishnan A., Lal K.K., Mohindra V., Sarkar U. K., Punia P., Singh, K.V., Bhat J.P. and Ayyappan S. 2015. DNA barcoding of Indian freshwater fishes. Mitochondrial DNA. doi. 10.3109/19401736.2015.1101540.published online 24 Dec 2015. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | DNA barcoding is a promising technique for species identification using a short mitochondrial DNA sequence of cytochrome c oxidase I (COI) gene. In the present study, DNA barcodes were generated from 72 species of freshwater fish covering the Orders Cypriniformes, Siluriformes, Perciformes, Synbranchiformes, and Osteoglossiformes representing 50 genera and 19 families. All the samples were collected from diverse sites except the species endemic to a particular location. Species were represented by multiple specimens in the great majority of the barcoded species. A total of 284 COI sequences were generated. After amplification and sequencing of 700 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.40%, 9.60%, 13.10%, and 17.16%, respectively. DNA barcode discriminated congeneric species without any confusion. The study strongly validated the efficiency of COI as an ideal marker for DNA barcoding of Indian freshwater fishes. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Mitochondrial DNA |
| NAAS Rating: | 5 |
| Volume No.: | 27 |
| Page Number: | 4510-4517 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.3109/19401736.2015.1101540 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/22948 |
| Appears in Collections: | FS-CIFRI-Publication |
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