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http://krishi.icar.gov.in/jspui/handle/123456789/25505| Title: | . A Modified Sequential Extraction Procedure for Glutenin (HMW & LMW),Gliadin and Other Proteins from Wheat Seed. |
| Other Titles: | Not Available |
| Authors: | Rajesh K. Gupta, Monika Dalal, G. Thomas, P.R. Choudhury and P.K.Mandal. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | National Research Centre on Plant Biotechnology, Pusa Campus, Indian Council of Agricultural Research (ICAR), New Delhi 110012, India |
| Published/ Complete Date: | 2016-08-14 |
| Project Code: | Not Available |
| Keywords: | Wheat seed, Gluten, SDS-PAGE, Quality, Celiac disease and antibody. |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The detection of Celiac disease responsible gluten protein fraction and development of antiglidin and antiglutenin antibody, require suitable and reliable extraction protocol. In the present study, the protocol is based on the sequential combination of extraction of albumin (water), globulin (0.5M NaCl), gliadin (50% 1-propanol) and glutenin (50% 1-propanol; 0.08 M Tris-HCl) from single seed. The composition of resulting protein fractions were analyzed by SDS-PAGE. The strength of our extraction protocol is that it starts with the pre-extraction to remove albumin and globulin. However, reducing agents had not used to be lethal for the T-cell cultures. Sequential extracted gluten proteins had less contamination of other fraction. It will be useful to investigate the toxicity of gluten (gliadin and glutenin) in improved wheat cultivars that affect the health of celiac patients. Consequently, it will be also fruitful for identification of wheat varieties on the basis of grain quality. |
| Description: | Not Available |
| ISSN: | 2278-3849 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Advances in Life Sciences |
| NAAS Rating: | Not Available |
| Volume No.: | 5(16) |
| Page Number: | 5959-5963 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/25505 |
| Appears in Collections: | CS-NIPB-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Rajesh Paper-2.pdf | 887.09 kB | Adobe PDF | View/Open |
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