KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/25958
Title: | Isolation and characterization of carotenoid producing bacteria from gut of Indian oil sardine Sardinella longiceps (Valenciennes, 1847) |
Other Titles: | Not Available |
Authors: | Susmitha, V. Anu Mathew Imelda Joseph |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology ICAR::Central Marine Fisheries Research Institute |
Published/ Complete Date: | 2017-11-21 |
Project Code: | Not Available |
Keywords: | Not Available |
Publisher: | ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch |
Citation: | Susmitha, V.,Anu Mathew and Imelda Joseph (2017) Isolation and characterization of carotenoid producing bacteria from gut of Indian oil sardine Sardinella longiceps (Valenciennes, 1847). In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 441-442 |
Series/Report no.: | Not Available; |
Abstract/Description: | In the present study, screening of bacteria from gut of Indian oil sardine Sardinella longiceps collected from Kanyakumari coast, Tamil Nadu, India, has led to the isolation of a yellow pigmented strain SR-G1. Cell morphology, motility and the occurrence of spores were examined by phase contrast microscopy. The isolate was Gram-positive, with irregular rods, nonmotile branched cocci with colonies on nutrient agar yellow, opaque, glistening, circular and low convex with entire margin. The pH of the medium and incubation temperature were found to be limiting factors in growth of the bacterial strain. The optimum temperature for growth was 28 degree celsius. Key biochemical reactions include positive for catalase and negative for oxidase. The strain was H 2S-producing, starch and gelatinhydrolyzing and alkali-tolerant. The isolate grew well in minimal media containing glucose, fructose and sucrose. The strain SR-G1 was identified as Microbacterium esteraromaticum based on the phenotypic characteristics and 16S rRNA sequence analysis. Extraction and separation of the bacterial carotenoid was carried out by a one step methanol of hexane extraction. The coloured supernatant in hexane solvent was analyzed by using UV- Visible Spectrophotometer from 350-550 nm range for detecting the lambda max . The bacterial pigment was identified using a combination of UV/visible spectral data and HPLC retention time as Neoxanthin. Neoxanthin is one of the major xanthophylls which are reported to be directly associated with reduction in the risk of cancers, cardiovascular disease, agerelated macular degeneration, and cataract formation. Hence, the potential of microbial producers as an alternative to chemical synthesis of xanthophylls could be further examined. |
Description: | Not Available |
ISBN: | 978-81-933623-1-0 |
Type(s) of content: | Other |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 441-442 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/25958 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
441_Isolation and characterization.pdf | 234.71 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.