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http://krishi.icar.gov.in/jspui/handle/123456789/26054
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sasi, S., Revathy, K.A. and Bhat, A. I | en_US |
dc.date.accessioned | 2019-11-30T11:22:02Z | - |
dc.date.available | 2019-11-30T11:22:02Z | - |
dc.date.issued | 2015-03-26 | - |
dc.identifier.citation | Sasi, S., Revathy, K.A. and Bhat, A. I. 2015. Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays. Journal of Plant Biochemistry and Biotechnology. 24: 466-469. | en_US |
dc.identifier.other | DOI 10.1007/s13562-015-0302-1 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/26054 | - |
dc.description | Not Available | en_US |
dc.description.abstract | A loop-mediated isothermal amplification (LAMP) and real-time LAMP based assays were developed for quick and sensitive detection of transgenic black pepper plants. Primers (six each) were designed based on the nucleotide sequence of two target regions [kanamycin and Cauliflower mosaic virus (CaMV) 35 S promoter] integrated into the genome of transgenic black pepper. The following conditions proved optimal for amplification: 6 mM of magnesium sulphate, 0.4 M of betaine and 1 h of reaction time. The assay successfully detected the transgenic plants whereas no cross-reaction was recorded with non-transgenic plants. The detection limit for LAMP was up to 10000 times that for conventional PCR and 1/1000 times that for real-time LAMP. The assays were validated by testing putative transformants of black pepper. The results presented clearly showed that LAMP and real-time LAMP assays developed in this study can provide a rapid and simple approach for screening transgenic black pepper plants. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | CaMV 35 S promoter | en_US |
dc.subject | detection | en_US |
dc.subject | kanamycin | en_US |
dc.subject | sensitivity | en_US |
dc.subject | validation | en_US |
dc.title | Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | BT/PR14813/AGR/02/761/2010 | en_US |
dc.publication.journalname | Journal of Plant Biochemistry and Biotechnology | en_US |
dc.publication.volumeno | 24 (4) | en_US |
dc.publication.pagenumber | 466-469 | en_US |
dc.publication.divisionUnit | Crop Protection | en_US |
dc.publication.sourceUrl | DOI 10.1007/s13562-015-0302-1 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Spices Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.77 | en_US |
Appears in Collections: | HS-IISR-Publication |
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