In vivo characterization of histological and immunological response of allergic protein of Metapenaeus dobsonii using an adjuvant free BALB/c mice model

Abstract

Shrimp allergy, a common form of food allergy is an adverse immunological response to shrimp proteins. BALB/c mice was sensitized by an adjuvant free oral administration of purified tropomyosin, from Metpenaeus dobsonii to characterize intestinal histological responses and immunological protein recognition pattern as it is unpractical in human subjects. Sensitized mice with higher dose of tropomyosin expressed symptoms of anaphylaxis including puffiness around eyes and snout, no activity, tremor and convulsion after challenge. The responses of high level of sera IgE, tropomyosin specific IgE and histamine in the treatment groups indicated the increased allergic reaction by ELISA. Sera IgE of sensitized mice exhibited a comparable recognition pattern to tropomyosin by immunoblotting similar to human subjects. Histological changes were comparatively highly affected in the intestinal area of duodenum in the sensitized mice. Hence BALB/c mice can be used as a suitable adjuvant free shrimp allergy model for immunotherapy tools.