KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/32250| Title: | Comparative analysis of genetic diversity among fluorescent pseudomonads using RAPD and ISSR fingerprinting |
| Other Titles: | Not Available |
| Authors: | Prabhukarthikeyan S.R., Keerthana U., Yadav Manoj Kumar and Raguchander T |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Rice Research Institute |
| Published/ Complete Date: | 2019-07-01 |
| Project Code: | Not Available |
| Keywords: | Fluorescent pseudomonads, Genetic diversity, ISSR, RAPD, 16S rRNA sequence, Turmeric ecosystem |
| Publisher: | Research Journal of Biotechnology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The genetic diversity of fluorescent pseudomonads associated with the turmeric (Curcuma longa L.) rhizosphere of Tamil Nadu, India was analyzed. Selected isolates were identified by ITS sequence analysis. Based on 16S rRNA sequence similarity, the isolates were identified as Pseudomonas fluorescens, P. stutzeri and Pseudomonas sp. The genetic variability and relationship among 15 fluorescent pseudomonads were analysed using 15 Random Amplified Polymorphic DNA (RAPD) and 13 Inter-Simple Sequence Repeat (ISSR) markers. Results revealed that ISSR markers were more efficient than the RAPD assay with regards to polymorphism detection. Also, total amplicons, the average number of polymorphic loci per primer, average polymorphic information content (PIC), marker index (MI) values and effective multiplex ratio (EMR) were more for ISSR than for RAPD. The similarity coefficients of fluorescent pseudomonads based on RAPD and ISSR markers ranged from 50.00 to 75.00 and 49.00 to 85.00 per cent respectively. Overall, we concluded that ISSR was found to be better than RAPD in assessing genetic diversity among fluorescent pseudomonads. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Research Journal of Biotechnology |
| NAAS Rating: | 4 |
| Volume No.: | 14(7) |
| Page Number: | 86-93 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/32250 |
| Appears in Collections: | CS-NRRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Parbhu RJB 2.pdf | 270.42 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.