Effect of 2, 4 D and EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032

Abstract

The present studies were carried out at Agricultural Research station, Perumallapalle to establish an efficient system for in vitro regeneration of sugarcane cultivar, Co86032 using chemical mutagens; Ethyl methane sulphonate (EMS) and 2, 4-dichlorophenoxy acetic acid (2, 4 D). Young leaf rolls were used as explants for callus induction on MS medium containing different concentrations of EMS (0.6 µM l-1, 0.8 µM l-1 and 1.0 µM l-1) and 2, 4-D (4mg l-1, 5mg l-1 and 6 mg l-1). Among the different concentrations, Co86032 had taken minimum number of days (11.89) for callus induction with 2, 4 D @ 4mg l-1, recording maximum mean callus size of 2.19 cm with high callus induction frequency (96.30%) and maximum mean number of explants inducing callus (2.78) in addition with superior response in shooting, rooting and hardening characters than EMS. EMS and 2, 4 Dat higher concentration (1.0 µM l-1 and 6mg l-1, respectively) had inhibitory effect on callusing, shooting and rooting and produced morphological traits variation which can be exploited in breeding programs