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http://krishi.icar.gov.in/jspui/handle/123456789/3483| Title: | Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
| Other Titles: | Not Available |
| Authors: | Akhilesh Dubey Mukunda Goswami Kamalendra Yadav Bhagwati S. Sharma |
| Published/ Complete Date: | 2014-01-01 |
| Project Code: | Not Available |
| Keywords: | Wallago attu Cell line Freshwater shark Characterization |
| Publisher: | Springer |
| Citation: | Dubey A, Goswami M, Yadav K, Sharma BS (2014). Development and characterization of a Cell line WAF from freshwater shark Wallago attu. Molecular Biology Reports. 41(2):915-24. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 _C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular markers. The cytogenetic analysis revealed a diploid count of 86 chromosomes at different passages. The origin of the cell lines was confirmed by the amplification of 547 and 654 bp sequences of 16S rRNA and cytochrome oxidase subunit I genes of mitochondrial DNA, respectively. WAF cells were characterized for their growth characteristics at different temperature and serum concentration. Epithelial morphology of the cell line was confirmed using immunocytochemistry. Further cell plating efficiency, transfection efficiency and viability of cryopreserved WAF cells was also determined. ytotoxicity and genotoxicity assessment of cadmium salts on WAF cells by MTT, NR and comet assay illustrated the utility of this cell line as an in vitro model for aquatic toxicological studies. The cell line will be further useful for studying oxidative stress markers against aquatic pollutants. |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Department of Biotechnology, Government of India |
| Language: | English |
| Name of Journal: | Molecular Biology Reports |
| NAAS Rating: | 7.4 |
| Volume No.: | 41(2) |
| Page Number: | 915-924 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1007/s11033-013-2936-1 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/3483 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| art%3A10.1007%2Fs11033-013-2936-1.pdf | 592.56 kB | Adobe PDF | View/Open |
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