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Title: | Molecular detection and in silico characterization of cold shock protein coding gene (cspA) from cold adaptive Pseudomonas koreensis |
Other Titles: | Not Available |
Authors: | Srikant Awasthi Anjney Sharma Pragya Saxena Jagriti Yadav K. Pandiyan M. Kumar Arjun Singh Hillol Chakdar Arpan Bhowmik Prem L. Kashyap Alok K. Srivastava Anil K. Saxena |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Bureau of Agriculturally Important Micro-organisms ICAR::Indian Institute of Wheat and Barley Research ICAR::Indian Agricultural Statistics Research Institute |
Published/ Complete Date: | 2019-03-27 |
Project Code: | Not Available |
Keywords: | Cold shock protein Pseudomonas koreensis Psychrophiles Gene expression Molecular modeling |
Publisher: | Journal of Plant Biochemistry and Biotechnology |
Citation: | Awasthi, S., Sharma, A., Saxena, P., Yadav, J., Pandiyan, K., Kumar, M., Singh, A., Chakdar, H., Bhowmik, A., Kashyap, P.L., Srivastava, A.K. and Saxena, A.K. Molecular detection and in silico characterization of cold shock protein coding gene (cspA) from cold adaptive Pseudomonas koreensis. Journal of Plant Biochemistry and Biotechnology. DOI 10.1007/s13562-019-00500-8 |
Series/Report no.: | Not Available; |
Abstract/Description: | Cold shock proteins (CSPs) are greatly conserved family of structurally related DNA binding proteins which are produced during temperature drift. A 213 bp long cspA gene was cloned and sequenced from Pseudomonas koreensis P2 in the present study. The expression analysis of the cspA showed 2.5 folds increase in the mRNA level at 15 C while the expression was almost on par at 30 C and 5 C indicating its role in moderately low temperature. In silico analyses of the gene showed that the gene codes for 7.69 kDa protein which was phylogenetically very similar to CspA present in Pseudomonads. Amino acid composition of the CspA from P. koreensis was different from that of mesophilic Pseudomonas and tiny/small amino varied significantly between CspA of cold adaptive and mesophilic species. The CspA from P. koreensis P2 contained RNP motifs involved in binding of DNA and RNA. Phylogenetic analyses revealed that the CspA protein of P. koreensis P2 was more close to CspA of distant subgroups of Pseudomonas like P. fluorescens and P. putida subgroup indicating a possible intra-specific gene transfer. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | The authors gratefully acknowledge the financial assistance under network project ‘Application of Microorganisms Agriculture and Allied Sectors (AMAAS)’ and ‘‘CRP Genomics’’ from Indian Council of Agricultural Research (ICAR), India. in |
Language: | English |
Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
NAAS Rating: | 6.77 |
Volume No.: | 28 |
Page Number: | 405-413 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://link.springer.com/article/10.1007/s13562-019-00500-8 https://doi.org/10.1007/s13562-019-00500-8 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/36248 |
Appears in Collections: | AEdu-IASRI-Publication |
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