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http://krishi.icar.gov.in/jspui/handle/123456789/36739| Title: | In Vitro Anti-Cervical Cancer Effect of Proteoglycans Isolated from Deep Sea Shark Cartilage by Inducing Apoptosis and Cell Cycle Arrest. |
| Other Titles: | Not Available |
| Authors: | Ajeeshkumar, K. K. Vishnu, K.V. Asha, K. K. Remyakumari, K. R. Reshma, J. Suseela Mathew |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Department of Biochemistry, SNGIST Arts and Science College (SNGIST ASc.), Affiliated to Mahatma Gandhi University, Kerala, India |
| Published/ Complete Date: | 2017-12 |
| Project Code: | Not Available |
| Keywords: | Proteoglycans Shark cartilage Apoptosis Cell cycle arrest |
| Publisher: | International Journal of Development Research |
| Citation: | Ajeeshkumar, K. K., Vishnu, K.V., Asha, K. K., Remyakumari, K. R., Reshma, J. And Suseela Mathew (2017) In Vitro Anti-Cervical Cancer Effect of Proteoglycans Isolated from Deep Sea Shark Cartilage by Inducing Apoptosis and Cell Cycle Arrest. International Journal of Development Research 7(12):17472-17478. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study was aimed at studying in vitro anti-cervical cancer effect of proteoglycans (PGs) isolated from deep sea shark. Anti-proliferative activity and apoptotic involvement were confirmed by following assays viz, the cell viability assay by MTT assay, morphological analysis and colony formation assay. The sample showed a significant cytotoxic activity for HeLa cells and results showed that the PGs at a concentration of 250μg/mL inhibit the colony formation capacity. Apoptosis induction was confirmed by the chromatin condensation assay by Hoechst 33342 nuclear staining. A number of apoptotic HeLa cells displayed a round and shrunken cell body and chromatin condensation inside the nucleus suggesting that PGs-induced apoptosis caused cell death. Apoptosis of HeLa cells was further verified by annexin V-FITC (fluorescein isothiocyanate) and PI (propidium iodide) staining by flow cytometry. PGs were found to induce apoptosis and the ratio of apoptotic cells were 30.5%. Cell cycle arrest at G1/M and G2/M phase confirmed the cell death due to cell cycle arrest. Our study determined that proteoglycans effectively induced apoptosis and caused cell cycle arrestestablishing their antiproliferative effect against cervical cancer. |
| Description: | Not Available |
| ISSN: | 2230-9926 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | International Journal of Development Research |
| Volume No.: | 7(12) |
| Page Number: | 17472-17478 |
| Name of the Division/Regional Station: | Biochemistry and Nutrition Division |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/36739 |
| Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IN VITRO ANTI-CERVICAL CANCER EFFECT OF PROTEOGLYCANS.pdf | 494.56 kB | Adobe PDF | View/Open |
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