Induction of non-protein thiols and phytochelatins by cadmium in Eichhornia crassipes

Abstract

Impact of root Cd concentration on production of cysteine, non-protein thiols (NP-SH), glutathione (GSH), reduced glutathione (GSSG), and phytochelatins (PCs) in Eichhornia crassipes exposed to different dilutions of brass and electroplating industry effluent (25%, 50%, and 75%), and synthetic metal solutions of Cd alone (1, 2.5, and 3.5 ppm) and with Cr (1ppm Cd þ 1ppm Cr, 2.5ppm Cd þ 3ppm Cr, and 3.5ppm Cd þ 4ppm Cr) was assessed in a 45 days study. Different treatments were used to understand and compare differential antioxidant defense response of plant under practical drainage (effluent) and experimental synthetic solutions. The production of NP-SH and cysteine was maximum under 2.5ppm Cd þ 3ppm Cr treatments i.e., 1.78 mmol/g fw and 288nmol/g fw, respectively. The content of GSH declined whereas that of GSSG increased progressively with exposure duration in all treatments. HPLC chromatograms revealed that the concentrations of PC2, PC3, and PC4 (248, 250, and 288nmol-SH equiv.g_1 fw, respectively) were maximum under 1ppm Cd, 1ppm Cd þ 1ppm Cr, and 2.5ppm Cd þ 3ppm Cr treatments, respectively. PC2, PC3, and PC4 concentrations increased with Cd accumulation in the range 812–1354 mg/g dry wt, 1354–2032 mg/g dry wt and 2032–3200 mg/g dry wt, respectively. Thus, the study establishes a direct proportionality relationship between concentration/length of phytochelatins and root Cd concentrations, upto threshold limits, in E. crassipes.