Molecular Identification of Dominant Cultivars of Soybean Using Simple Sequence Repeat Markers

Abstract

Not AvailableMolecular identity of dominant cultivars ofsoybean needs to complement the morphological descrip-tors, which are insufficient and influenced by environment,for protecting the interest of breeders and maintaining thegenetic purity. In the present investigation, genomic DNAof six commercial Indian soybean varieties viz. JS95-60,JS93-05, NRC7, JS97-52, NRC37, JS335 was amplifiedusing ten simple sequence repeat (SSR) markers, whichwere selected based upon the relatively higher polymorphicinformation content (PIC) value observed after polymor-phism survey of 46 soybean genotypes through 58 SSRmarkers. PCR analysis showed nine polymorphic SSRprimers which could differentiate unambiguously all thesix varieties. Maximum similarity was observed betweenNRC7, JS93-05 and JS95-60. Two unique alleles amplifiedin each of the two varieties namely JS335 and NRC37 andfour unique alleles in JS97-52 unambiguously differenti-ated them from the remaining varieties. Morphologicallysimilar varieties JS93-05 and JS95-60 could be differenti-ated distinctly by primer pair Satt181, while NRC7 couldbe distinguished from both JS93-05 and JS95-60 throughSct_199. Results also showed that these nine polymorphicmarkers could distinguish the dominant cultivars from sixnon-trading varieties, which further confirmed the utility ofnine SSR primers for protecting the breeders’ rights andmaintaining the purity of dominant cultivars during seedproduction programme