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http://krishi.icar.gov.in/jspui/handle/123456789/39826
Title: | Coliphage test: a quick and easy method to detect faecal pollution in water and fish |
Other Titles: | Not Available |
Authors: | Rao, B.M. Surendran, P.K. |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
Published/ Complete Date: | 2003-01-01 |
Project Code: | Not Available |
Keywords: | Coliphage test Escherichia coli Faecal pollution |
Publisher: | Society of Fisheries Technologists(India),Cochin |
Citation: | Rao, B.M., and Surendran, P.K. (2003). Coliphage test: a quick and easy method to detect faecal pollution in water and fish. In : Proceedings of the Symposium on Seafood Safety - Status and Strategies .Surendran, P.K. et. al (Eds.), pp 556-561, Society of Fisheries Technologists (India), Cochin. |
Series/Report no.: | Not Available; |
Abstract/Description: | Coliphages are viruses that infect Escherichia coli. Coliphages have been frequently proposed as rapid and inexpensive indicators of faecal pollution. This study describes a simple method termed as coliphage test for the detection of coliphage in water and fish. Coliphage test involves the assay of coliphages by the single agar layer method using nutrient agar medium. The number of plaque forming units was higher on nutrient agar (445 pfu. 100ml^-1) than on other bacteriological media. The result of coliphage test was obtained within 6 hours. Faecal coliforms and Escherichia coli survive freezing temperatures only upto 6 weeks of storage whereas coliphages resisted freezing temperature and survived even after 10 weeks of storage (maximum period tested). Frozen fish and shrimp from processing plants had low levels of coliphages (0-12 pfu.g^-1), faecal coliforms (0-2.5 .g^-1) and E. coli (0-0.9 .g^-1) whereas market samples had high levels of faecal coliforms (140+ .g^-1), E. coli (140+ .g^-1) and coliphages (96-158 pfu.g^-1). In the processing plant samples, 7% were positive for E. coli and 27% positive for faecal coliforms whereas 47% of the samples were positive for coliphages. The result suggested that coliphages were better indicators of faecal pollution in frozen samples than faecal coliforms and E. coli as they survive for a longer period at -20degreeC. The advantages of the coliphage test were that the test was easy to perform, result was faster (4-6 hours) and the test was less expensive. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Proceedings |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 556-561 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/39826 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
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Coliphage Test A Quick and Easy Method to Detect Faecal Pollution in Water and Fish.pdf | 3.39 MB | Adobe PDF | View/Open |
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