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http://krishi.icar.gov.in/jspui/handle/123456789/41631| Title: | Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton) |
| Other Titles: | Not Available |
| Authors: | Chaturvedi Preeti Dhanik Meenakshi Pande Veena Pande Amit |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Directorate of Cold Water Fisheries Research |
| Published/ Complete Date: | 2016-01-01 |
| Project Code: | Not Available |
| Keywords: | Liver-expressed antimicrobial peptide (LEAP), Antimicrobial peptides (AMPs), Hepcidin, Innate immunity, Microbes |
| Publisher: | Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases |
| Citation: | Chaturvedi, P., Dhanik, M. and Pande, A. (2016). Identification and in-silico analysis of hepcidin from Tor Putitora (Hamilton). Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 37(2):67-76. DOI:10.5958/0974-0147.2016.00013.1 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Antimicrobial peptides extensively expressed in vertebrates, and invertebrates have assorted mechanisms of action and protect against pathogens. Liver-expressed antimicrobial peptide (LEAP) is a multifunctional cysteine-rich peptide that provides protection against infectious pathogens besides iron regulation. We have identified and cloned cDNA encoding LEAP from Tor putitora (TP-LEAP) as 440 bp fragment, with an open reading frame (ORF) of 279 nucleotides. The ORF encodes a pre-propeptide of 93 and a mature peptide of 25 amino acids. 3D model of the mature TP-LEAP, predominantly a β sheet, was generated and validated. Motif analysis and docking of TP-LEAP with iron transporter ferroportin revealed that the disulfide bonds are responsible for a stable structure besides interaction with ferroportin. Arg8 and Cys11, of mature TP-LEAP, formhydrogen bonds with Asn332 and Thr323 with bond lengths of 2.48Å and 2.55Å, respectively. TP-LEAP was phylogenetically closely related to Schizothorax richardsonii and Cyprinus carpio. |
| Description: | Not Available |
| ISSN: | 0970-9320 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases |
| Volume No.: | 37(2) |
| Page Number: | 67-76 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI:10.5958/0974-0147.2016.00013.1 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/41631 |
| Appears in Collections: | FS-DCWFR-Publication |
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