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http://krishi.icar.gov.in/jspui/handle/123456789/42495| Title: | Development and characterization of microsatellite markers from enriched genomic libraries in safflower (Carthamus tinctorius L.). |
| Other Titles: | Not Available |
| Authors: | Usha Kiran B Mobeen Shaik Kadirvel P Mukta N S. Senthilvel |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Oilseeds Research Centre for Cellular and Molecular Biology |
| Published/ Complete Date: | 2019-01-01 |
| Project Code: | Not Available |
| Keywords: | Genetic diversity genomic library molecular markers oilseed crop polymorphism |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Microsatellite markers are the ideal genetic markers for crop improvement. In this study, we developed and characterized a set of 200 genomic SSR markers in safflower, an important oilseed crop of the world. A microsatellite enriched genomic library was constructed from an Indian cultivar, A-1. A total of 750 SSR-positive clones was generated of which 617 were identified as unique sequences and sequencing of them revealed 238 SSR motifs. The SSRs are validated in a panel of 24 genotypes and found 42 polymorphic markers. The number of alleles ranged from 2 to 4 with an average of 2.7 with polymorphic markers. The polymorphic information content (PIC) value ranged from 0.08 to 0.61 with an average of 0.33. A dendrogram based on the polymorphic SSR loci clearly indicated the genetic relationships among genotypes. The reported SSR markers would be useful for characterization of genetic diversity and trait mapping purposes in safflower |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Research Journal of Biotechnology |
| NAAS Rating: | 4 |
| Volume No.: | 14(12) |
| Page Number: | 14-20 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/42495 |
| Appears in Collections: | CS-IIOR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 33 . researchJournalofBiotechnology.pdf | 413.74 kB | Adobe PDF | View/Open |
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