KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4317| Title: | Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues |
| Other Titles: | Methods in Molecular Biology |
| Authors: | Mulpuri Sujatha K. B. R. S. Visarada |
| Published/ Complete Date: | 2012-01-01 |
| Project Code: | 101-2 |
| Keywords: | Biolistics Gene gun Castor Embryo axes Embryogenic calli Microprojectile bombardment Plant transformation |
| Publisher: | Springer nature |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Particle bombardment/biolistic delivery is a very popular method of genetic transformation of diverse targets including cells and intact tissues. Delivery of DNA through particle bombardment is genotype and species independent, nevertheless, an ef fi cient protocol for large-scale generation of transgenic plants through embryogenic tissues with a high ( ³ 80%) shoot regeneration ef fi ciency is a prerequisite. Young embryogenic tissues or multiple shoot buds in early stages of induction are the most suited target tissues for recovery of transgenic plants. We describe the protocol for delivery of foreign genes using particle delivery system (Biorad gene gun, PDS-1000/He) in to the meristematic tissues of embryonic axes derived from mature seeds of castor. With the optimized physical and biological parameters, putative transformants were obtained at a frequency of 1.4% through particle gun bombardment of castor embryo axes. Also, transformation of embryogenic calli of sorghum using particle in fl ow gun (PIG) is described. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Book chapter |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | 940 |
| Page Number: | 27-44 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | https://link.springer.com/protocol/10.1007%2F978-1-62703-110-3_3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4317 |
| Appears in Collections: | CS-IIOR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.