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http://krishi.icar.gov.in/jspui/handle/123456789/43606| Title: | Identification of Heat-Related ESTs in Moth Bean Through Suppression Subtraction Hybridization |
| Other Titles: | Not Available |
| Authors: | Karun Gurjar Sakshi Rampuria Uma Joshi Paramita Palit K. V. Bhatt T. Mohapatra Ramavtar Sharma |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Arid Zone Research Institute |
| Published/ Complete Date: | 2014-06-01 |
| Project Code: | Not Available |
| Keywords: | Moth bean SSH cDNA library Sequence analysis HSPs Semi-qRT-PCR assay |
| Publisher: | Not Available |
| Citation: | 66. Karun Gurjar & Sakshi Rampuria & Uma Joshi & Paramita Palit & K. V. Bhatt & T. Mohapatra & Ramavtar Sharma. 2014. Identification of Heat-Related ESTs in Moth Bean through Suppression Subtraction Hybridization. Appl Biochem Biotechnol 173 (8), 2116-2128. DOI 10.1007/s12010-014-1011-2 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Abstract Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant genotype RMO-40 to identify genes expressing under delayed response to elevated temperature. Heat induction was carried out by exposing 14-day-old seedlings to elevated temperature of 42 °C for 30 min. A total of 125 unigenes (33 contigs and 92 singletons) were derived by cluster assembly and sequence alignment of 200 ESTs; out of 125 unigenes, 21 (16 %) were found to be novel to moth bean. Gene ontology functional classification terms were retrieved for 98 (78.4 %) unigenes of |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Applied Biochemistry and Biotechnology |
| NAAS Rating: | 8.28 |
| Volume No.: | 173 (8) |
| Page Number: | 2116-2128 |
| Name of the Division/Regional Station: | Div.III (PI&PM) |
| Source, DOI or any other URL: | 10.1007/s12010-014-1011-2 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/43606 |
| Appears in Collections: | NRM-CAZRI-Publication |
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