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http://krishi.icar.gov.in/jspui/handle/123456789/44431
Title: | CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome |
Other Titles: | Not Available |
Authors: | Kutubuddin A. MollaYinong Yang |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-NRRI |
Published/ Complete Date: | 2020-07-01 |
Project Code: | Not Available |
Keywords: | CRISPR-Cas, Base Editing, Locus in Rice |
Publisher: | Springer |
Citation: | https://doi.org/10.1007/978-1-0716-0616-2_4 |
Series/Report no.: | Not Available; |
Abstract/Description: | Base editing is a recently developed technology that enables us to install point mutation in the genome precisely. The fusion of nucleotide deaminase with Cas9 nickase gives rise to base editors. Like the conventional CRISPR-Cas9 technology, a single-guide RNA can direct a base editor to a particular locus to change one nucleotide to another. In this chapter, we describe a stepwise protocol to perform targeted base editing experiments in rice plants. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Book |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome |
Page Number: | 51-62 |
Name of the Division/Regional Station: | CROP IMPROVEMENT DIVISION |
Source, DOI or any other URL: | https://link.springer.com/protocol/10.1007/978-1-0716-0616-2_4 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/44431 |
Appears in Collections: | CS-NRRI-Publication |
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