Histological studies of the intestine in threatened Asian catfish (Clarias batrachus) fingerlings fed with animal or plant origin protein blended with glucosamine.

Abstract

In the present study a 12-week feeding trials was conducted with animal or plant protein ( in combination with Glucosamine source @ 0.0, 0.5%, 5.0% and 10.0%) with Asian Catfish, Clarias batrachus (av. wt. 2.2+0.009 to 2.6+0.03 g) to evaluate the effects of proteins of different origin, blended with glucosamine, on intestinal tissues. Intestine of C. batrachus fed with natural food (NATFO, F7) showing normal architecture of intestine with circular muscles, longitudinal muscles, serosa and villi. Intestine of fish fed with P:A: G::0:100:0.5 feed ( F1) and P:A:G::0:100:5.0 feed (F2) showing normal appearance of circular muscles, longitudinal muscles, serosa and villi. Intestine of fish fed with Intestine of fish fed with P:A:G::0:100:10.0 feed (F3) depicting normal appearance of circular muscles, longitudinal muscles, serosa and villi. Fusion of few villi is recorded. Intestine of fish fed with P:A:G::100:0:0.5 feed (F4) showing appearance of spaces between circular muscles and base of villi. Normal circular muscles, longitudinal muscles and serosa are seen. Intestine of fish fed with P:A:G::100:0:5.0 feed (F5) showing elongated lumen in villi. Circular muscles, longitudinal muscles and serosa are normally seen. Intestine of fish fed with P:A:G::100:0:10.0 feed (F6). Showing elongated lumen in villi. Circular muscles, longitudinal muscles and serosa are seen normally. Showing some detachment of base of villi observed. Results indicate that animal protein diets rich with glucosamine showing normal intestinal tissues architecture than in plant protein fed fishes. The plant protein fed fishes showed an alteration in the intestinal architecture in this threatened fish. However, the results suggests that supplementation of glucosamine has no direct relation with the histological alterations in the fish intestinal tissues.