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http://krishi.icar.gov.in/jspui/handle/123456789/46879| Title: | Expression of Rhizobium meliloti symbiotic promoters in Azorhizobium caulinodans. |
| Other Titles: | Not Available |
| Authors: | Das Avijit, Khanuja SPS and Lodha ML |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Indian Agricultural Research Institute |
| Published/ Complete Date: | 1997-01-01 |
| Project Code: | Not Available |
| Keywords: | oxygen sensitive, Azorhizobium caulinodans, azide tolerance, expression, symbiotic promoter,Rhizobium meliloti, nitrogen fixation |
| Publisher: | Society for Plant Biochemistry and Biotechnology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Plasmids containing Rhizobium meliloti symbiotic promoters P1 (promoter of nifHDK) and P2 (promoter of fixABCX) when mobilized into the cells of Azorhizobium caulinodans strain IRBG 46 showed strong expression of these promoters under free-living microaerobic as well as symbiotic conditions. Under free-living conditions microaerobiosis (3% or less O2) was found to be sufficient to activate these promoters; expression being higher at 1% than at 3% O2 concentration. Under symbiotic conditions the expression was much more stronger-with bacteroids in stem nodules showing higher expression than those in root nodules. Under both the conditions expression of the promoters in the native R. meliloti strain Rm102F34 was lower than that in the A. caulinodans strain IRBG 46. The results suggest a functional homology of these promoters in the heterologous background of A. caulinodans. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Experimental Biology |
| Journal Type: | Monthly |
| NAAS Rating: | 6.78 6.78 |
| Impact Factor: | 0.783 |
| Volume No.: | 35 |
| Page Number: | 1331 – 1335 |
| Name of the Division/Regional Station: | Division of Biochemistry |
| Source, DOI or any other URL: | https://pubmed.ncbi.nlm.nih.gov/9567770/ |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/46879 |
| Appears in Collections: | AEng-NINFET-Publication |
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