Green pea, Pisum sativum leaf meal with supplemented exogenous cellulase and xylanase can completely replace de-oiled rice bran (DORB) in the diet of Labeo rohita (Hamilton, 1878) fingerlings

Abstract

Green pea, Pisum sativum leaf meal (PSLM) was evaluated by conducting a 60-day feeding trial in the diet of Labeo rohita fingerlings. Six iso-nitrogenous (310 g CP/kg) and isocaloric (14 MJ DE/kg) diets viz. Control (0 g PSLM/kg), LM150 (150 g PSLM/kg), LM300 (300 g PSLM/kg), CCX (control + 1 g cellulase and xylanase, CX mixture/kg), LM150CX (LM150 + 1 g CX/kg) and LM300CX (LM300 + 1 g CX/kg) were prepared. Two hundred and seventy acclimated fish (5.06 ? 0.08 g) were randomly distributed in six experimental groups in triplicate with the stocking density of 15 fish per tank and the fish of each experimental tank were fed with respective diet at satiation level. The specific growth rate, protein efficiency ratio, apparent dry matter digestibility coefficient, amylase activity and the activities of muscle aspartate aminotransferase and alanine aminotransferase of LM150CX group were the highest among the experimental groups. However, LM300CX and control groups exhibited significantly higher (p < 0.05) values of these parameters than other groups except LM150CX group. Hepatic catalase and superoxide dismutase activities of LM150CX group were the lowest. Moreover, LM300CX and control groups showed significantly lower (p < 0.05) activities of these enzymes than other dietary groups except LM150CX group. The groups fed with leaf meal and exogenous enzyme showed enhanced hepatic IGF-1 expression. In conclusion, dietary inclusion of PSLM at 300 g/kg with supplementation of CX (1:1) at 1 g/kg could replace DORB completely in the diet of L. rohita without compromising the growth performance.