Genome-wide In Silico Identification of Transcriptional Regulators Controlling the Response to Salt Stress in Soybean (Glycine max (L.) Merr.)

Abstract

Comprehensive characterization of networks of gene expression regulators is key challenge of functional genomics. Soybean genomic sequences can be used to frame model for binding sites of fundamental transcription factors, and RNA seq data. It has been manifested that the reverse genetics approach can reveal gene regulator networks in soybean, which explains regulatory mechanisms for salt response from expression of gene patterns. In this study, the sequences of protein is functionally validated salt tractable genes is used to key out soybean orthologous with the help of ‘blastP’ search against Glycine max genome database. Thirty- eight salt responsive genes allocate among 39 cis-acting elements of soybean is elucidated by the ‘PlantCARE’ database. Gene promoter analysis of salt tractable genes indicated the presence of biotic and abiotic stress responsive, light, hormone, plant tissues specific and gene specific cis-regulatory elements in soybean. A total, 38 salt tractable genes in soybean is entrusted for GO terms based on the ‘Ensembl Plants’ database. These GO terms are sum up into the three main categories (biological process, cellular component, and molecular function) and 62 sub-categories. These GO annotations comprise a general profile signature of gene expression for salt tractable genes in soybean. Our results explained about the identified soybean salt tractable genes differentially respond in plant growth, development and gene regulation during biotic and abiotic stress conditions. The data obtained from this study contribute to a better enlightening of the role of the regulatory and functional pathway of salt tolerant genes in soybean.