KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50784| Title: | Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH |
| Other Titles: | Not Available |
| Authors: | Ravindra Kumar Vishwamitra Singh Baisvar Basdeo Kushwaha Gusheinzed Waikhom Labee Thoidingjam S Sanjabihari Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources, Lucknow Institute of Bioresources and Sustainable Development, Imphal |
| Published/ Complete Date: | 2020-03 |
| Project Code: | Not Available |
| Keywords: | Glossogobius giuris Giemsa AgNOR CMA3 FISH 18S rDNA |
| Publisher: | Springer India |
| Citation: | Kumar, R., Baisvar, V.S., Kushwaha, B., Waikhom, G., Thoidingjam, L. and Singh, S.S., 2020. Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 90(1), pp.221-226. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The Glossogobius giuris (Gobiidae), tank goby a species of goby fish, is native mainly to freshwater and estuaries and has importance in the aquarium trade. It is classified as least concern under IUCN Red List. Live individuals of this species were collected from Loktak Lake in Manipur for cytogenetic investigation to reveal the nucleolar organizer region (NOR) through silver nitrate (AgNO3) and chromomycin A3 (CMA3) staining as well as DAPI staining and single-color fluorescence in situ hybridization (FISH) using 18S rDNA sequence as probe. Analysis of more than 50 metaphase chromosome complement (obtained from colchicine–potassium chloride–Carnoy’s fixation–Giemsa staining procedures) showed the presence of 46 diploid chromosome numbers with all telocentric having fundamental arm number as 46 without any heteromorphic pair. One pairs of silver-stained NORs, situated terminally on the telocentric chromosome, were observed. Similarly, one pair of CMA3-positive sites were observed on the chromosome that suggested abundance of GC-rich repetitive DNAs in this region. One pair of 18S rDNA positive sites was observed on the telocentric chromosomes using FISH. These karyological features can be useful markers in cytotaxonomy and conservation of this species. |
| Description: | Not Available |
| ISSN: | 2250-1746 (web) 0369-8211 (print) |
| Type(s) of content: | Research Paper |
| Sponsors: | Department of Biotechnology, Ministry of Science and Technology, Government of India, for financial support through Project Sanction No. BT/13/NE/TBP/2010 |
| Language: | English |
| Name of Journal: | Proceedings of the National Academy of Sciences, India Section B: Biological Sciences |
| Journal Type: | The research/review papers of different fields of science, e.g. Agriculture Sciences (Agriculture, Animal Husbandry, Fisheries, Forestry, Agric. Toxicology, Soil Science, Plant Protection, Post Harvest Technology, and Agricultural Engineering), Animal Sciences (Structural, Developmental, Functional, Genetical, Ecological, Behavioural, Taxonomical and Evolutionary Aspects), Biochemistry, Biophysics, Biotechnology (including Molecular and Cell Biology, Structural and Functional Studies, Microbiology and Immunology), Medical & Forensic Sciences |
| Impact Factor: | 0.396 |
| Volume No.: | 90 (1) |
| Page Number: | 221-226 |
| Name of the Division/Regional Station: | Molecular Biology and Biotechnology |
| Source, DOI or any other URL: | 10.1007/s40011-019-01084-y https://link.springer.com/article/10.1007/s40011-019-01084-y |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50784 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.