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http://krishi.icar.gov.in/jspui/handle/123456789/50814| Title: | Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5 |
| Other Titles: | Not Available |
| Authors: | V Mageshwaran S Walia K Annapurna |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute for Research on Cotton Technology |
| Published/ Complete Date: | 2012-01-01 |
| Project Code: | Not Available |
| Keywords: | Antibacterial activity Characterization Lipopeptide Paenibacillus polymyxa Purification Xanthomonas campestris pv. phaseoli |
| Publisher: | Springer |
| Citation: | Mageshwaran V, Suresh Walia and K Annapurna. 2012. Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5. World Journal of Microbiology and Biotechnology.28: 909-917 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | An antibacterial metabolite was isolated from Paenibacillus polymyxa HKA-15, a soybean bacterial endophyte. The purification of the crude metabolite from Paenibacillus polymyxa HKA-15 was done by column chromatography. In TLC, a spot with an Rf value of 0.86 (±0.02) from the purified fraction showed bioactivity against Xanthomonas campestris pv. phaseoli M-5. In SDS-PAGE, the purified antibiotic was separated in the molecular weight range of 3.5 kDa. The exact molecular weight of the active compound was identified as 1,347.7 Da using MS-MS analysis. Infra red spectrum and 1 H NMR analysis showed the presence of amino acids and fatty acids in the active compound. The characterization of the antibacterial compound revealed its lipopeptide nature. In an agar diffusion assay, the crude metabolite showed a broad spectrum of activity, being able to inhibit the growth of the fungal pathogen, Rhizoctonia bataticola, Macrophomina phaseolina and Fusarium udum. A stronger inhibition was observed against bacterial pathogens viz., X. campestris pv.phaseoli M-5, X. campestris pv. phaseoli CP-1-1, Xanthomonas oryzae, Ralstonia solanacearum and Micrococcus luteus. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | World Journal of Microbiology and Biotechnology |
| NAAS Rating: | 8.48 |
| Volume No.: | 28 |
| Page Number: | 909-917 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI 10.1007/s11274-011-0888-y |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50814 |
| Appears in Collections: | CS-NBAIMO-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| world j mic biotech.pdf | 431.59 kB | Adobe PDF | View/Open |
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