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http://krishi.icar.gov.in/jspui/handle/123456789/50892| Title: | LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane. |
| Other Titles: | Not Available |
| Authors: | Shingote PR, Amitha Mithra SV, Sharma P, Devanna NB, Arora K, Holkar SK, Khan S, Singh J, Kumar S, Sharma TR, Solanke AU |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR - National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110012, India ICAR-Indian Institute of Sugarcane Research, Lucknow 226002, India |
| Published/ Complete Date: | 2019-01-21 |
| Project Code: | Not Available |
| Keywords: | Genetic fidelity testing, IRAP, ISSR, Phylogeny, Somaclonal variation, Sugarcane |
| Publisher: | Not Available |
| Citation: | Shingote PR, Amitha Mithra SV, Sharma P, Devanna NB, Arora K, Holkar SK, Khan S, Singh J, Kumar S, Sharma TR, Solanke AU (2019) LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane. Molecular Breeding 39 (2), 25 (DOI: 10.1007/s11032-019-0931-5) |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Testing the genetic fidelity of micropropagated clones with DNA markers could curtail the losses caused by somaclonal variation in sugarcane tissue culture industry. Evaluation of 32 inter-retrotransposon amplified polymorphism (IRAP) markers and 100 inter-simple sequence repeats (ISSRs) in 47 sugarcane accessions identified 20 (8 IRAPs and 12 ISSRs) polymorphic markers representing 98 loci. IRAP system was superior to ISSR in terms of marker index (2.68 as against 1.76), resolving power (2.85 as against 1.98), and polymorphic loci per assay (6 compared to 4.1), except mean polymorphic information content (0.31 and 0.34). Further evaluation of the 20 polymorphic markers in testing the genetic fidelity of micropropagated sugarcane clones identified a variant clone by three primers of ISSR and one IRAP marker (UBC810, UBC813, UBC840, and LTR6149 + 3’LTR). The unique amplicons from the somaclonal variant were validated by sequencing. Based on the number of bands amplified and proportion of polymorphic loci, a set of six ISSR and four IRAP markers has been recommended to test clonal fidelity for sugarcane tissue culture industry. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Molecular Breeding |
| NAAS Rating: | 8.15 |
| Impact Factor: | 2.149 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI: 10.1007/s11032-019-0931-5 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50892 |
| Appears in Collections: | CS-NIPB-Publication |
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