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http://krishi.icar.gov.in/jspui/handle/123456789/50899| Title: | Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene. |
| Other Titles: | Not Available |
| Authors: | Ramakrishna Ch, Sonam, Mohanty S, Reddy N, Padaria JC and Solanke AU |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR - National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110012, India School of Biotechnology, KIIT University, Bhubaneswar, Odisha 751024, India All India Coordinated Research Project on Small Millets, ICAR, GKVK, Bangalore 560065, India |
| Published/ Complete Date: | 2017-09-21 |
| Project Code: | Not Available |
| Keywords: | Finger millet, bZIP transcription factor, ER stress, Heat stress, Conserved gene |
| Publisher: | Not Available |
| Citation: | Ramakrishna Ch, Sonam, Mohanty S, Reddy N, Padaria JC and Solanke AU (2017). Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene. 3 Biotech. (doi:10.1007/s13205-017-0984-2) |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we report isolation and characterization of EcbZIP17, a group B bZIP transcription factor from a climate smart cereal, finger millet (Eleusine coracana L.). The genomic sequence of EcbZIP17 is 2662 bp long encompassing two exons and one intron with ORF of 1722 bp and peptide length of 573 aa. This gene is homologous to AtbZIP17 (Arabidopsis), ZmbZIP17 (maize) and OsbZIP60 (rice) which play a key role in endoplasmic reticulum (ER) stress pathway. In silico analysis confirmed the presence of basic leucine zipper (bZIP) and transmembrane (TM) domains in the EcbZIP17 protein. Allele mining of this gene in 16 different genotypes by Sanger sequencing revealed no variation in nucleotide sequence, including the 618 bp long intron. Expression analysis of EcbZIP17 under heat stress exhibited similar pattern of expression in all the genotypes across time intervals with highest upregulation after 4 h. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Biotech-3 |
| NAAS Rating: | 7.8 |
| Impact Factor: | 2.406 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | doi:10.1007/s13205-017-0984-2 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50899 |
| Appears in Collections: | CS-NIPB-Publication |
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