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http://krishi.icar.gov.in/jspui/handle/123456789/50931| Title: | PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates |
| Other Titles: | Not Available |
| Authors: | Mishra, S. S. Brahmane, Manoj Mishra, S.K.. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Inland Fisheries Research Institute |
| Published/ Complete Date: | 2009-01-01 |
| Project Code: | Not Available |
| Keywords: | PCR DNA |
| Publisher: | Not Available |
| Citation: | Mishra, Sudhansu S. & Brahmane, Manoj & Mishra, S.K.. (2009). PCR detection and DNA restriction profile analysis of vibrio parahaemolyticus isolates. Indian Veterinary Journal. 86. 229-233. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | V. parahaemolyticus isolates from fish and shrimp P. monodon were screened using PCR and 623 bp product could be visualized in positive cases. The selected isolates were further characterized using protein profile analysis on 11% SDS-PAGE and DNA restriction profile analysis. Variable protein bands on SDS-PAGE indicated antigenic variation among V. parahaemolyticus isolates. EcoRI, BamHI and Haelll were found unsuitable for DNA-RE analysis but Hind III digestion revealed numerous DNA fragments in the range of 15.0 kb to 0.5 kb with variation among isolates. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | The Indian Veterinary Journal |
| NAAS Rating: | Not available |
| Volume No.: | 86 |
| Page Number: | 229-233 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50931 |
| Appears in Collections: | FS-CIFRI-Publication |
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