Functional Food Quality of Curcuma caesia, Curcuma zedoaria and Curcuma aeruginosa Endemic to Northeastern India

Abstract

Curcuma spp. (Zingiberaceae) is one of the significant ingredients in food and traditional medicines. The current study was to investigate health - benefits of the rhizomes of endemic Curcuma caesia, Curcuma zedoaria and Curcuma aeruginosa using in vitro antioxidant, antiinflammatory and human tumor cell proliferation inhibitory activities. Among these, C. caesia (black turmeric) showed the best overall biological activities based on [3 - (4, 5 - dimethylthiazole - 2 - yl) - 2,5 - diphenyltetrazolium bromide] (MTT) and lipid peroxidation (LPO), cyclooxygenase (COX - 1 and - 2) enzymes, and tumor cell growth inhibitory assays. The hexane and methanolic extracts of C. caesia (CCH and CCM) showed LPO inhibition by 31 and 43 %, and COX - 2 enzyme by 29 and 38 %, respectively, at 100 g/ml. Eleven terpenoids were isolated and identified. The MTT antioxidant assay revealed that the extracts of three Curcuma spp. at 250 g/ml and isolates at 5 g/ml demonstrated activity comparable to positive controls vitamin C and t - butyl hydroquinone (TBHQ) at 25 g/ml. The extracts inhibited LPO by 40 % at 250 g/ml whereas pure isolates 111 by about 20 %. The extracts and isolates inhibited COX - 1 and - 2 enzymes between the ranges of 356 and 530 %, respectively. The in vitro biological activity exhibited by the extracts and isolates of C. caesia rhizome further supported its use in traditional medicine.