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http://krishi.icar.gov.in/jspui/handle/123456789/52612| Title: | Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties |
| Other Titles: | Not Available |
| Authors: | Sakthivel K, Shobha Rani N, Pandey M, Sivaranjani AKP, Neeraja CN, Balachandran SM, Madhav MS, Viraktamath BC, Prasad GSV and Sundaram RM |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2009-05-01 |
| Project Code: | Not Available |
| Keywords: | Oryza sativa Rice Fragrance Aroma badh2 Functional marker |
| Publisher: | Not Available |
| Citation: | Sakthivel, K., Shobha Rani, N., Pandey, M.K. et al. Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties. Mol Breeding 24, 185–190 (2009). https://doi.org/10.1007/s11032-009-9283-x |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Fragrance development in rice has been reported due to a 8-bp deletion in the exon 7 of badh2 gene located on Chromosome 8S. Multiplex markers targeting the functional InDel polymorphism was earlier reported for genotyping fragrance trait, but the marker was observed to be inconsistent and difficult to use. We have developed a simple, co-dominant, functional marker for fragrance trait, which can be resolved in an agarose gel and validated in Basmati and non-Basmati aromatic rice varieties and in a mapping population segregated for fragrance trait. The marker targets the InDel polymorphism in badh2 gene and amplifies 95 and 103 bp fragments in fragrant and non-fragrant genotypes, respectively. The newly developed marker was highly efficient in discriminating all fragrant and non-fragrant genotypes and showed perfect co-segregation with the trait of fragrance in the mapping population. We recommend the use of this simple, low-cost marker in routine genotyping for fragrance trait in large scale breeding materials and germplasm. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Department of Biotechnology, Government of India Indian Council of Agricultural Research |
| Language: | English |
| Name of Journal: | Molecular Breeding |
| NAAS Rating: | 8.15 |
| Impact Factor: | 2.149 |
| Volume No.: | 24 |
| Page Number: | 185–190 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s11032-009-9283-x |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52612 |
| Appears in Collections: | CS-IIRR-Publication |
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