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http://krishi.icar.gov.in/jspui/handle/123456789/52620| Title: | Molecular fingerprinting of the elite, fine-grain type rice cultivar, Samba Mahsuri (BPT 5204) and assessment of genetic purity in seed-lots of the variety using SSR markers |
| Other Titles: | Not Available |
| Authors: | Arunakumari K, Durga Rani Ch. V, Vanisree S, Sivaramakrishnan S, Balachandran SM and Sundaram RM |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2011-04 |
| Project Code: | Not Available |
| Keywords: | Genetic purity SSRs Samba Mahsuri DUS Multiplex PCR |
| Publisher: | Not Available |
| Citation: | Kumari, K & Durgarani, Ch.V & Satturu, Vanisree & Sivaramakrishnan, Siva & Balachandran, S. & Sundaram, Raman. (2011). Molecular fingerprinting of the elite, fine-grain type rice cultivar, Samba Mahsuri (BPT 5204) and assessment of genetic purity in seed-lots of the variety using SSR markers. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Samba Mahsuri is an elite, fine-grain type rice variety which is grown extensively in India. In order to fingerprint Samba Mahsuri and to differentiate it from 26 other closely related fine-grain type varieties, we utilized a total of 113 SSR markers, which included 87 hyper variable SSRs and 26 (GATA)n motif specific SSRs distributed uniformly across the rice genome. Twenty-six SSRs were found to be polymorphic among the rice varieties analyzed, amplifying a total of 74 alleles with an average of 2.84 alleles per marker. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped the 27 rice varieties into five clusters. Samba Mahsuri and another elite fine-grain type variety, Sona Mahsuri (BPT 3291) were observed to be grouped in the same cluster. A twin-marker combination JGT11-16.3 + RM19426 could unambiguously differentiate Samba Mahsuri from other fine-grain type varieties through multiplex-PCR. To utilize these SSR markers specific for Samba Mahsuri effectively for detection of impurities in Samba Mahsuri, a rapid and cost effective two-dimensional bulked DNA sampling strategy was designed and validated. |
| Description: | Not Available |
| ISSN: | 2250-3153 |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | International Journal of Scientific Research |
| NAAS Rating: | Not Available |
| Impact Factor: | 0 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | www.ijsrp.org/research_paper_dec2011/ijsrp-dec-2011-13.pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52620 |
| Appears in Collections: | CS-IIRR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ijsrp-dec-2011-13.pdf | 493.08 kB | Adobe PDF | View/Open |
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