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http://krishi.icar.gov.in/jspui/handle/123456789/52823| Title: | Production of doubled haploids from rice hybrid KRH-2 through anther culture and their evaluation for agro-morphological traits |
| Other Titles: | Not Available |
| Authors: | Kulkarni SR, Ulaganathan K, Sundaram RM, HariPrasad AS, Abdul Fiyaz R and Balachandran SM |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2020-05-30 |
| Project Code: | Not Available |
| Keywords: | Rice androgenesis Callus induction Green plant regeneration KRH-2 morpho-agronomic evaluation |
| Publisher: | Not Available |
| Citation: | Kulkarni SR, Ulaganathan K, Sundaram RM, HariPrasad AS, Abdul Fiyaz R and Balachandran SM (2020). Production of doubled haploids from rice hybrid KRH-2 through anther culture and their evaluation for agro-morphological traits. Journal of Rice Research 13: 33-50 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study was aimed to optimize conditions of anther culture and production of doubled haploids of a popular rice hybrid, KRH-2. Immature panicles collected from field grown donor plants were pre-treated at 8°C for eight days and plated on four different basic media for callus induction with different hormonal concentrations and supplements. N6 media with maltose as the carbon source, supplemented with 2 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg L-1 of kinetin and 10 mg L-1 of silver nitrate (designated as N6-4 medium) was found to give the highest callus induction (22.4%). The compact calli, which were transferred to MS regeneration medium supplemented with 2.5 mg L-1 of benzyl amino purine (BAP), 0.5 mg L-1 of kinetin and 1.5 mg L-1 of naphthalene acetic acid (NAA) recorded a good regeneration (25.4%). A total of 125 stable, doubled haploid lines (DHLs) were produced through spontaneous doubling of KRH-2 microspores. The lines showed significant variability with respect to key agro-morphological traits |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Rice Research |
| NAAS Rating: | 4.05 |
| Impact Factor: | 0 |
| Volume No.: | 13(1) |
| Page Number: | 33-50 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://sarr.co.in/Journals/2020-1/volume-13,%20June-2020,chapter-4.pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52823 |
| Appears in Collections: | CS-IIRR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| volume-13, June-2020,chapter-4.pdf | 2.09 MB | Adobe PDF | View/Open |
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