KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/52836| Title: | Assessment of genetic purity of bulked‐seed of rice CMS lines using capillary electrophoresis |
| Other Titles: | Not Available |
| Authors: | Anupama K, Pranathi K and Sundaram RM |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2020-05-01 |
| Project Code: | Not Available |
| Keywords: | rice CMS lines genetic purity capillary electrophoresis |
| Publisher: | Not Available |
| Citation: | Anupama K, Pranathi K and Sundaram RM (2020). Assessment of genetic purity of bulked‐seed of rice CMS lines using capillary electrophoresis. Electrophoresis (published online, doi: https://doi.org/10.1002/elps.201900429). |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Genetically pure cytoplasmic male sterile line (A-line) is essential to generate pure hybrid seeds in order to harness the yield heterosis in rice. Conventionally, seed purity testing is carried by grow-out test, which has many limitations. Seed purity assessments based on molecular markers reduce the time required for analysis significantly. However, it is very tedious as at least 200–400 seeds/seedlings are needed to be analyzed individually. An assay based on bulked-seed and molecular markers will be an ideal system. Keeping these points in view, in the present study, a co-dominant mitochondrial marker was used to test the purity of bulked parental line (A-line) seed utilizing CE system in a genetic analyzer. The results indicate that this method is very simple, accurate, and can be used to test purity of large number of samples rapidly in a cost-effective way compared to grow-out test and conventional molecular marker analysis. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Electrophoresis |
| Impact Factor: | 3.081 |
| Volume No.: | 41(20) |
| Page Number: | 1749-1751 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1002/elps.201900429 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52836 |
| Appears in Collections: | CS-IIRR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.