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http://krishi.icar.gov.in/jspui/handle/123456789/53088| Title: | Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance |
| Other Titles: | Not Available |
| Authors: | Joseph, T.C. Anbu Rajan, L. Nirmala Thampuran Roswin James |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
| Published/ Complete Date: | 2010-09 |
| Project Code: | Not Available |
| Keywords: | E. coli Compatible solutes Trehalose Salt stress otsA otsB |
| Publisher: | Springer |
| Citation: | Joseph, T. C., Anbu Rajan, L., Nirmala Thampuran and Roswin James (2010) Functional characterization of Trehalose biosynthesis genes from E. coli: An osmolyte involved in stress tolerance, Mol. Biotechnol., 46(1): 20–25. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Trehalose (1-a-D-glucopyranosyl-1-a-D-glucopyranoside), a non-reducing disaccharide is a major compatible solute, which maintains fluidity of membranes and protects the biological structure of organisms under stress. In this study, trehalose-6-phosphate synthase (otsA) and trehalose-6-phosphate phosphatase (otsB) genes encoding for trehalose biosynthesis from Escherichia coli was cloned as an operon and expressed in E. coli M15(pREP4). The recombinant E. coli strain showed a threefold increase in the activity of otsBA pathway enzymes, compared to the control strain. The transgenic E. coli accumulated up to 0.86 mg/l of trehalose. The sequence of otsA and otsB genes reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. |
| Description: | Not Available |
| ISSN: | 1073-6085 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Molecular Biotechnology |
| Journal Type: | International Journal |
| NAAS Rating: | 8.02 |
| Impact Factor: | 2.02 |
| Volume No.: | 46(1) |
| Page Number: | 20–25 |
| Name of the Division/Regional Station: | Microbiology, Fermentation and Biotechnology Division |
| Source, DOI or any other URL: | https://doi.org/10.1007/s12033-010-9259-4 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/53088 |
| Appears in Collections: | FS-CIFT-Publication |
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