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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/53288
Title: | Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans |
Other Titles: | Not Available |
Authors: | Anburajan, L. Joseph, T.C. Nirmala Thampuran Roswin James Visvanathan, C. Bansal, K. C. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Water Technology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India |
Published/ Complete Date: | 2008-07-16 |
Project Code: | Not Available |
Keywords: | B. halodurans Compatible solutes Salt stress Ectoine genes Osmoregulation |
Publisher: | Springer |
Citation: | Anburajan, L., Joseph, T. C., Nirmala Thampuran, Roswin James, Visvanathan, C. and Bansal, K. C. (2008) Characterisation and phylogenetic analysis ecotine biosynthesis genes from Bacillus halodurans. Archives Microbiol. 190: 481-487. |
Series/Report no.: | Not Available; |
Abstract/Description: | Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was confirmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved. |
Description: | Not Available |
ISSN: | 0302-8933 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Archives of Microbiology |
Journal Type: | International Journal |
NAAS Rating: | 7.88 |
Impact Factor: | 1.88 |
Volume No.: | 190 |
Page Number: | 481-487 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s00203-008-0397-z |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/53288 |
Appears in Collections: | FS-CIFT-Publication |
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