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http://krishi.icar.gov.in/jspui/handle/123456789/56338
Title: | Influence of pH on the solubility and conformational characteristics of muscle proteins from mullet (Mugil cephalus) |
Other Titles: | Not Available |
Authors: | Mukund Mohan Dhanya Ramachandran Sankar, T.V. Anandan, R. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
Published/ Complete Date: | 2007-07 |
Project Code: | Not Available |
Keywords: | Fish muscle pH Solubility Reactive sulphydryls Surface hydrophobicity SDS-PAGE |
Publisher: | Elsevier |
Citation: | Mukund Mohan, Dhanya Ramachandran, Sankar, T. V. and Anandan, R. (2007) Influence of pH on the solubility and conformational characteristics of muscle proteins from mullet (Mugil cephalus). Process Biochem. 42: 1056-1062. |
Series/Report no.: | Not Available; |
Abstract/Description: | Mullet (Mugil cephalus) muscle homogenates were adjusted to different pH ranging from 2 to 12 and the proteins extracted were evaluated for changes in solubility and conformational characteristics viz. surface hydrophobicity and reactive sulphydryl groups. Altering the pH of muscle homogenate to acidic or alkaline increased protein solubility. The hydrophobicity of the proteins increased on exposure to extreme pH indicating unfolding. The reactive sulphydryl groups decreased at acidic and alkaline pH with the lowest at pH 4. When the pH of the muscle homogenates was brought back to the original pH (6.3), the protein solubility was found to decrease. Reactive sulphydryl groups and ANS hydrophobicity of the proteins increased on readjusting the pH resulting in a molten-globule state. The electrophortogram of the samples corresponded well with the observations. Alterations in functional properties of these modified proteins are an area of interest for commercial application. |
Description: | Not Available |
ISSN: | 1359-5113 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Process Biochemistry |
Journal Type: | International Journal |
NAAS Rating: | 8.95 |
Impact Factor: | 2.95 |
Volume No.: | 42(7) |
Page Number: | 1056-1062 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1016/j.procbio.2007.04.005 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/56338 |
Appears in Collections: | FS-CIFT-Publication |
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