Microbe-mediated alleviation of drought stress and acquisition of phosphorus in great millet (Sorghum bicolour L.) by drought-adaptive and phosphorus-solubilizing microbes

Abstract

Among the extreme habitats, drought is most harsh abiotic stress affecting growth, development and productivity of crops. Plants also face limitations of certain nutrients such as phosphorus required for different physiological and metabolic activities. Stress-adaptive phosphorus-solubilizing microbes in rhizospheric soil can help plants to combat water scarcity and overcome the problem of phosphorus unavailability to plant systems. The present investigation deals with the isolation of drought stress adaptive and P-solubilizing microbes from rhizospheric soil of different cereals and pseudocereals and their role in mitigation of drought stress in great millet. A total of 193 rhizospheric microbes were isolated and screened for their capability to solubilize phosphorus under drought stress. Twenty isolates exhibited P-solubilizing attribute under drought stress, which were further screened for plant growth promoting (PGP) traits such as solubilization of zinc and potassium; production of Fe-chelating compounds, indole acetic acid, hydrogen cyanide and ammonia. On basis of multifunctional PGP traits, two efficient and potential microbes were evaluated for PGP in great millet in vitro under green house with different water regimes. The isolates were found to be efficient in terms of enhancing accumulation of different osmolytes such as glycine betaine, proline, sugars, increased chlorophyll content, and decreasing lipid peroxidation. The isolates were identified by 16S/18S rRNA gene sequencing as Streptomyces laurentii EU-LWT3-69 and Penicillium sp. strain EU-DSF-10. To best of our knowledge Streptomyces laurentii has been reported first time as PGP and drought adaptive bacterium. PGP drought-adaptive phosphorus solubilizers could be used as bioinoculants for crops under water scarcity ecosystems.