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http://krishi.icar.gov.in/jspui/handle/123456789/64581
Title: | Modelling of PrnD protein from Pseudomonas fluorescens rajNB11 and its comparative structural analysis with prnD proteins expressed in Burkholderia and Serratia |
Other Titles: | Not Available |
Authors: | Singh RN, Singh RP, Sharma A and Saxena AK |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-NATIONAL BUREAU OF AGRICULTURALLY IMPORTANT MICROORGANISMS KUSHMAUR MAU NATH BHANJAN 275103 |
Published/ Complete Date: | 2016-01-01 |
Project Code: | Not Available |
Keywords: | Pseudomonas fluorescens, pyrrolnitrin, cloning, protein modeling |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | yrrolnitrin is produced by Pseudomonas fluorescens and plays an important role in control of pathogenic fungi. The prnABCD gene cluster codes for enzymes involved in biosynthesis of Pyrrolnitrin. Among the four genes, prnD is very important as it codes for arylamine N-oxygenase, the only biochemically characterized oxygenase involved in oxidation of the amino group of aminopyrrolnitrin to a nitro group to form pyrrolnitrin. A strain of Pseudomonas fluorescens RajNB11 efficient for antifungal activity was found to produce pyrrolnitin. The prnD gene product of P. fluorescens RajNB11, that catalyses the final step of production of pyrrolnitrin, was characterized in the present study with regards to its stability and catalytic activity. The prnD gene was amplified from RajNB11, sequenced, translated and 3-D modeled to protein structure. The modeled protein of test organism was compared to PrnD proteins of Burkholderia sp. and Serratia sp. The protein from P. fluorescens was found to be basic, hydrophilic with higher thermal stability and easily separated after expression and purification. The protein of P. fluorescens was found to have better folded structure, more serine residues in the active site, better active site properties and iso-electric point value in comparison to PrnD proteins of Burkholderia and Serratia. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Turkish Journal of Biology |
NAAS Rating: | 7.56 |
Volume No.: | 40 |
Page Number: | 623- 633 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/64581 |
Appears in Collections: | CS-NBAIMO-Publication |
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