KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/64795| Title: | Addition of Cholesterol-Loaded Cylodextrin Improves Cryosurvival of Buffalo Spermatozoa |
| Other Titles: | Not Available |
| Authors: | Kumar A. Prasad J. K. MAHAK SINGH Rajoriya J.S |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Research Complex for NEH Region ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2019-01-01 |
| Project Code: | Not Available |
| Keywords: | Buffalo bull sperm Cholesterol Cyclodexterin Cryopreservation Post-thaw |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Cryopreservation helps in prolonging the storage of semen but freezing and thawing damage the sperm because of alteration in the structure and physiology of the sperm cell. Buffalo bull sperm were treated with different levels of cholesterol loaded cyclodextrin (CLC) and frozen in tris egg yolk glycerol diluents to determine the best suitable concentrations of CLC that benefits Buffalo sperm cryosurvival. Semen collected from Buffalo bull was diluted to 120×106 spermatozoa/ml by tris diluents and divided into four groups; control (without CLC), 0.5, 1.0 and 2.0 mg CLC/ml of diluted semen as Group I, II, III and IV respectively. At prefreeze stage, the sperm treated with 2.0 mg CLC showed significantly (P<0.01) higher values for progressive motility, viability, acrosomal integriry and HOS response. No significant difference was observed between groups I, II and III in seminal parameters at pre-freeze stage except HOS response. After thawing, significantly (P<0.01) higher percentage of progressive motility, viability, acrosomal integrity and HOS reponse was observed in spermatozoa treated with 2.0 mg CLC than any other treatment groups including control. Group I and II showed no significant (P<0.01) difference in post-thaw seminal parameters indicating that 0.5 mg CLC is not sufficient to prevent the cryodamage of Buffalos sperm during cryopreservation. In conclusion, addition of CLC @ 2.0 mg to semen extender for freezing of Buffalo spermatozoa reduces the cryoinjuries. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Veterinary Clinical Science |
| Journal Type: | Peer Reviewed |
| Volume No.: | 7 |
| Page Number: | 59-63 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/64795 |
| Appears in Collections: | NRM-RCNR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.