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http://krishi.icar.gov.in/jspui/handle/123456789/6602| Title: | Biochemical screening of genotypes for trypsin inhibitor content and morpho-molecular characterization of soybean [Glycine max (L.) Merrill.] |
| Other Titles: | Not Available |
| Authors: | 10. Verma, K., Talukdar, A., Shivakumar, M., Kumar, B., Lal, SK., Srivastava, N Sapra RL Harish G.D and Vanishree Girmilla |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR- Indian Institute of Soybean Research ICAR:Indian Agricultural Research Institute |
| Published/ Complete Date: | 2015-11-01 |
| Project Code: | Not Available |
| Keywords: | Soybean, trypsin inhibitor, simple sequence repeats, morphological diversity, genetic diversity |
| Publisher: | Not Available |
| Citation: | Verma, K., Talukdar, A., Shivakumar, M., Kumar, B., Lal, SK., Srivastava, N Sapra RL Harish G.D and Vanishree Girmilla (2015). Biochemical screening of genotypes for trypsin inhibitor content and morpho-molecular characterization of soybean [Glycine max (L.) Merrill.]. Indian J. Genet. 75(4): 490-496 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A set of 101 diverse soybean genotypes were biochemically analyzed for trypsin inhibitor (TI) contents, and were characterized with morphological and molecular markers. The TI content in the seeds ranged from 14.65 to 175.52 mg g–1 soymeal. The Indian soybean varieties were found to contain higher amount of TI (58.79-126.78 mg g–1 soymeal) while PI542044, an exotic germplasm was found to contain the least (14.65 mg g–1 soymeal). Morphological characterization with 16 qualitative and quantitative traits revealed significant variations and grouped the genotypes in to three major groups with overall mean dissimilarity value of 0.30. Genetic diversity study conducted with 100 simple sequence repeat (SSR) markers detected higher level of polymorphism (71%). Of the total 221 alleles amplified, 65 were rare (frequency <0.2). The mean polymorphism information content (PIC) of the markers was 0.27 while the value for gene diversity was 0.44. UPGMA-based cluster analysis grouped the genotypes into four major clusters of which I, II and III were occupied by genotypes containing higher and moderate level of TI. PI542044, which was free from Kunitz trypsin inhibitor (KTI), grouped singly. Such genotype would be suitable for use in breeding program directed towards development of varieties with zero KTI |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Genetics and Plant Breeding |
| NAAS Rating: | 6.55 |
| Volume No.: | 75(4) |
| Page Number: | 490-496 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | 10.5958/0975-6906.2015.00078.4 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/6602 |
| Appears in Collections: | CS-DSBR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Molecular Diversity Analysis.pdf | 1.39 MB | Adobe PDF | View/Open |
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