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http://krishi.icar.gov.in/jspui/handle/123456789/66733| Title: | Real time PCR assay based on Topoisomerase II gene for detection of Fusarium udum. |
| Other Titles: | Not Available |
| Authors: | Yadav MK, Babu BK, Saxena AK, Singh BP, Singh K and Arora D.K. 2010 |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-NATIONAL BUREAU OF AGRICULTURALLY IMPORTANT MICROORGANISMS KUSHMAUR MAU NATH BHANJAN 275103 |
| Published/ Complete Date: | 2010-01-01 |
| Project Code: | Not Available |
| Keywords: | Housekeeping genes SYBR green Melting curve Standard curve |
| Publisher: | Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Fusarium wilt is an important soilborne disease of pigeonpea, caused by Fusarium udum. In this study, we have designed a real-time PCR assay for the detection of Fusarium udum from infected pigeonpea plants. Based on Topoisomerase-II gene sequence data from Fusarium udum and other related Fusarium species, a pair of primer was designed. The species-specific primers were tested in real-time PCR SYBR green assay. No increasing fluorescence signals exceeding the baseline threshold was observed with tested microbes, except Fusarium udum DNA. A single dissociation peak of increased fluorescence was obtained for the specific primers at melting temperature of 81.25°C. The real-time PCR showed a lowest detection of 0.1 pg genomic DNA. The assay was more sensitive, accurate and less time consuming for detection of Fusarium udum in infected plants root. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Mycopathologia (Mycopathologia et Mycologia Applicata) |
| NAAS Rating: | 8.45 |
| Volume No.: | 171 |
| Page Number: | 373-381 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/66733 |
| Appears in Collections: | CS-NBAIMO-Publication |
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