Identification and Molecular Characterization of Castellaniella ginsengisoli Isolated from Sugarcane-Wheat Cropping System

Abstract

An increasing interest has emerged with respect to the importance of microbial diversity in soil habitats. The extent of the diversity of microorganisms in soil is seen to be critical to the maintenance of soil health and quality, as a wide range of microorganisms is involved in important soil functions. Most soil microorganisms are still unknown. A Gramnegative, rod-shaped, non-spore-forming bacterium, designated as strain P2, was isolated from the soil of the wheat ratooning field of ICAR-Indian Institute of Sugarcane Research, Lucknow, 16S rRNA gene sequence analysis showed that the isolate was closely related to species of the genus Castellaniella. Castellaniella ginsengisoli DCY36 was shown to be the most closely related (99% 16S rRNA gene sequence similarity), followed by Castellaniella ginsengisoli strain MN ZOO (99%). Castellaniella ginsengisoli, RNA secondary structure prediction was also been performed by RNA Vienna RNA Web Services.